ABSTRACT
The COVID-19 pandemic is the biggest global health crisis in years. China is the first market primarily affected by the COVID-19 pandemic, with unprecedented lockdown measures bringing real estate and other economic activities to a standstill. This study has two objectives: (1) to identify the risks critical to the risk management of commercial real estate (CRE) development projects based on the project life cycle stages and (2) to identify the stages most affected by the COVID-19 pandemic and the risk factors at different stages. Three rounds of the Delphi study were conducted with nine experts involved in the construction project. The findings indicate that the construction, lease and sale phases are prone to significant risks. Additionally, the analytic hierarchy process (AHP) identified ‘health and safety risk' as the most critical risk factor during the construction phase and ‘marketing and payback risk' as the most critical risk factor during the lease and sale phase. This study enhanced the effectiveness of risk management practices for implementing CRE development projects in China.
ABSTRACT
PurposeThe ongoing pandemic has gravely affected different facets of society and economic trades worldwide. During the outbreak, most manufacturing and service sectors were closed across the globe except for essential commodities such as food and medicines. Consequently, recent literature has focused on studying supply chain resilience and sustainability in different pandemic contexts. This study aims to add to the existing literature by exploring the economic, environmental and societal aspects affecting the food supply chain and assessing the impact of COVID-19 on food sustainability.Design/methodology/approachA survey method has been adopted with a questionnaire instrument investigating the role of technology, government policies, geopolitics and intermediaries on sustainable organisational management. A five-point Likert scale (i.e. 1 = strongly disagree;5 = strongly agree) is used to evaluate the responses. The findings are based on 131 responses from entry-level workers and senior executives of different food supply chains across Asia and Europe. The data has been analysed to derive insights into the impacts of this pandemic.FindingsThe survey concludes with the significant impact of COVID-19 on the three pillars of sustainability, i.e. economic, social and environmental dimensions. The empirical analysis shows digitalisation and its applications help mitigate the negative effect of COVID-19 on sustainability. In addition, the supportive government policies and intermediatory interventions were helpful in improving sustainability at each level.Research limitations/implicationsThe findings have implications for businesses and policymakers. Companies can learn from the advantages of digitalisation to counter the challenges imposed by the pandemic or similar situations in the future in maintaining the sustainability of their supply chains. Managers can also learn the importance of effective organisational management in driving sustainability. Finally, policymakers can devise policies to support businesses in adopting sustainable practices in their supply chains.Originality/valueThis study adds to the limited literature exploring the impact of COVID-19 on food supply chain sustainability through the triple bottom line lens. To the best of the authors' knowledge, this is also one of the first empirical studies to examine the effect of technology, government and organisational management practices on the sustainability of food supply chains.
ABSTRACT
BACKGROUND: Small, non-coding microRNAs, usually of 20-25 nucleotides, are known to regulate the post-transcriptional gene expression, which has a significant role in human biological processes, including immune-biogenesis, homeostasis and infection control as differential expression of such miRNAs is responsible for fine-tuning the organismic development. METHODS: A search of bibliographic databases was carried out with a focused question on microRNA-Disease Prediction. A deductive qualitative content analysis approach was further employed to assess the research's overall outcomes, review articles on prediction tools in miRNA-Diseases, and analyse the interventions. RESULTS: Diagnosis and therapeutics of diseases and miRNA prediction methods hold importance in identifying the regulatory mechanisms. Collections of efficient miRNA prediction methods to identify miRNA-mRNA-disease regulatory relationships have been presented through this review, consolidating the potential of miRNAs as a diagnostic and prognostic biomarker of multiple diseases, including COVID-19. CONCLUSIONS: The role of miRNA in the aetiology and pathogenesis of wide-range of pathologies, including viral, bacterial to chronic diseases such as cancer, is quite feasible through the modern tools in bioinformatics which has been elaborated focusing upon miRNA-disease prediction methods and their application potential establishing miRNAs as a robust and reliable biomarker in clinico-medical studies.
ABSTRACT
[...]it becomes essential to understand the PM aspects in the face of emergency situations such as COVID-19. Since the seminal article by Benita Beamon proposing new performance measures for evaluating supply chain performance, the literature has evolved. [...]the guest editors would also like to thank the authors for their contributions and for choosing our special issue as a relevant platform to communicate their research works. The insights drawn from this SI will provide them with effective guidance to help them design, implement and improve performance measurement systems capable of effectively measuring different supply chain processes and issues during unexpected and disruptive events.Table 1 Articles published in this special issue Article Title Purpose 1 Airline catering supply chain performance during pandemic disruption: a Bayesian network modelling approach This study aims to consider the impact of implementing Bayesian network (BN) modelling to measure SC performance in the airline catering during the pandemic context 2 The role of Industry 4.0 technologies on performance measurement systems of supply chains during global pandemics: an interval-valued intuitionistic hesitant fuzzy approach This study aims to investigate supply chain performance measurement systems (SCPMSs) that are suitable and applicable to evaluate SC performance during unexpected events such as global pandemics. [...]it considers the contribution of Industry 4.0 Disruptive Technologies (IDTs) to implement SCPMSs during such black swan events 3 A systematic literature review on supply chain resilience in SMEs: learnings from COVID-19 pandemic This paper presents the state-of-art literature on supply chain resilience in SMEs in the context of the coronavirus (COVID-19) pandemic and provides a comprehensive view of insights gained and gaps identified and suggests potential areas of future research 4 A proposed circular-SCOR model for supply chain performance measurement in the manufacturing industry during COVID-19 This study aims to determine which supply chain performance criteria come to the fore for the company under consideration to accelerate the transformation into high performance and circularity in supply chains, considering that the ability to analyse supply chain performances and ensure circularity in supply chains has become one of the factors whose importance has increased rapidly with COVID-19 5 How do food supply chain performance measures contribute to sustainable corporate performance during disruptions from the COVID-19 pandemic emergency?
ABSTRACT
PURPOSE: Chemiluminescence Immunoassay (CLIA) is high throughput, rapid diagnostic test which has recently come up for the detection of SARS-CoV-2 antigen. The present study evaluated performance of CLIA antigen test in nasopharyngeal swab samples stored at different temperatures for 7 days to simulate the transport conditions and transit time across the country from remote peripheral laboratories to central facilities. MATERIALS AND METHODS: Limit of detection (LOD), sensitivity and specificity of VITROS® SARS-CoV-2 antigen assay was determined using Real-time reverse transcriptase PCR (rRT-PCR) confirmed SARS-CoV-2 positive and negative samples. To detect the effect of storage temperatures on VITROS ®SARS-CoV-2 antigen results, samples were stored at 4 â°C, 25 â°C & 37 â°C for 7 days followed by detection of SARS-CoV-2 nucleocapsid antigen and compared with N-gene rRT-PCR. RESULTS: The VITROS® SARS-CoV-2 antigen test was found to have a sensitivity and specificity of 78.9% and 100% respectively with high sensitivity of 88.1% for samples with Ct â< â30. The LOD of VITROS assay was equivalent to 3800 copies of RNA per reactions as compared to 72 copies per reaction for rRT-PCR. We observed that more than 80% of samples with <30 Ct values could be detected by VITROS SARS-CoV-2 antigen assay at day 7 even when stored at 37 â°C. For samples with Ct values between 26 and 30, on day 7 the positivity rate of N-antigen at 4 â°C was 90.9% and 37 â°C was 63.6%. CONCLUSIONS: CLIA testing can be carried out for the detection of SARS-CoV-2 N-protein in NP-swab samples transported in cold chain even with 7 days transit time, particularly for Ct â< â30 samples which represents cases with higher transmissibility. As drop in positivity for VITROS assay was lower as compared to rRT-PCR on day 7 in cold chain-maintained samples, the assay can be useful to screen samples received from remote peripheral areas before performing rRT-PCR.
Subject(s)
COVID-19 , Humans , COVID-19/diagnosis , Luminescence , SARS-CoV-2 , Temperature , Nasopharynx , Immunoassay , Sensitivity and SpecificityABSTRACT
Background: COVID-19 severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was declared Pandemic by the World Health Organization on January 30, 2020. Vaccination represents the best possibility to resolve this pandemic. The current global challenge is the immunization against the SARS-CoV-2. However, the adverse events following immunization (AEFI) of the corona vaccine remains unclear. Aim and Objectives: This observational study aims to represent an accountable data of the AEFI between Covaxin and Covishield in North Indian population. Materials and Methods: The hospital-based prospective and observational study was employed from January 2021 to December 2021 for detecting and monitoring of AEFI in adults. All population vaccinated either covishield or covaxin with both doses were enrolled in the study as targeted population. Post-vaccination vaccinated population were telephonic follow-up with prior consent. Results: A total of 1015 vaccinated individuals were included in this study for assessment of AEFI. After statistical analysis of AEFI between both vaccination at 24 h P = 0.13, 3–7 days 0.4 and complete AEFI P = 0.06 observed. There is no association that was found significant P < 0.05 with the incidence of AEFI. Conclusion: The short-term outcome has not attribute any serious AEFI. This study demonstrated that both vaccines were well-tolerated and safe in generalized population. [ FROM AUTHOR] Copyright of Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research) is the property of Journal of Cardiovascular Disease Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)
ABSTRACT
Purchase intention has been acknowledged as an important factor influencing businesses' sales performances and sustainability. Thus, finding factors that influence purchase intention is essential to all relevant businesses. Based on the current importance of purchase intention to businesses, the main objective of this research was to investigate how country of origin, brand image, and perceived value influenced intentions to purchase COVID-19 medicines among Thai consumers. To accomplish this objective, researchers created a Google Form to survey 862 people around Thailand. However, researchers found only 653 valid data, which were analyzed through the structural equation model. The research findings revealed that perceived COVID-19 medicine value increased once the values of country of origin and brand image were highly considered by consumers. At the same time, consumers attempted to buy COVID-19 medicines for their COVID-19 treatments if the products contained high country of origin and perceived values. Finally, the perceived value was found to be a full mediator between brand image and purchase intention. In comparison, despite country of origin and perceived value being the significant factors in purchase intention, consumers' intention to purchase COVID-19 medicines depended significantly on the degree of the consumers' perceived value because perceived value contributed the highest impact on purchase intention. These results revealed that COVID-19 medicines were highly valued by many consumers because these medicines could help prevent heavy illness in consumers. As a result, consumers had a higher intention to purchase these medicines for their future COVID-19 treatments.
Subject(s)
Antiviral Agents , COVID-19 Drug Treatment , COVID-19 , Consumer Behavior , Humans , Intention , Southeast Asian People , ThailandABSTRACT
The vaccine development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is primarily focused on structure of the spike (S) protein. The heavy glycosylation of S with flexible hinges at stalk shields from antibodies. The flexible nature of hinges may be one of the important factors which are responsible for binding the odorant receptor of those neurons which are responsible for the loss of smell in patients with COVID-19 infection. In this study strong and stable bond formation results from reaction between R:14: Trp and Phe at the residue, the expected outcome of this research may help in designing a novel vaccine that targets the flexible hinges of SARS-CoV-2. The difference in the conformational structure of S protein and their binding with the odorant receptor in COVID-19 are prime factor for the loss of smell and taste in patients supported by the concept of Antigen (epitope) Antibody interaction by stable formation of Hydrogen bond among odorant receptor and the S protein. The flexibility of structural proteins determines if the antibodies or other defense proteins produced are homologous participating in antigen antibody reaction thus maintaining the most neutralization sensitive epitope to produce the new vaccine strain or in isolating most effectively neutralizing human mABs. Molecular and atomic level details potentiate the design and screening of small molecules that can inhibit the fusion at entry level or at odorant receptors and potentially be used in prevention and treatment of infection particularly when formulated as nasal drops, paving a new approach for pharmacologist in treatment of COVID-19 infection.
ABSTRACT
SARS-CoV-2 has mutated many times among different populations. We analyzed wild-type spike protein and 18 different variants of SARS-CoV-2 spike protein known until the beginning of 2022 (alpha, beta, B.1.429, B.1.616, B.1.620, B.1.617.3, C.1.2, delta, epsilon, eta, gamma, iota, kappa, lambda, mu, omicron, theta, and zeta) for their interaction with 16 phytocompounds and remdesivir, resulting into 425 combinations. The largest number of mutations has been reported in the omicron followed by delta variant. However, the virulence of the delta variant has been reported higher as compared to omicron. Mutations at a few locations (D215G, K417N, E484K, N501Y, D614G, and P681H) were common in most of the variants. 3 D structures of all the 18 spike proteins were created using SWISS-MODEL to test the binding affinities with caffeine theophylline, emodin, vitexin, berberine, curcumin, piperine, quercetin, artemisinin, carvacrol, capsaicin, tetrahydrocannabinol, cannabidiol, α- pinene, ß- pinene and gingerol. Phytocompounds and mutant variants were prepared using AutoDock 4.2.6 software. Binding affinities of the selected phytocompounds with the different mutant spike proteins were achieved using AutoDock Vina. Out of all combinations investigated, the best binding affinities were observed with 3 variants of SAR-CoV-2 with 5 phytocompounds along with remdesivir. The range of best binding energies varied from -9.1 to -8.0 kcal/mol. Further, MD simulation was done for selected 9 phytocompound-spike mutant complexes for analyzing the stability of interactions for 100 ns. ADMET studies via ProTox-II and SwissADME displayed that phytocompounds are safe and less toxic in comparison to remdesivir.Communicated by Ramaswamy H. Sarma.
ABSTRACT
[...]none of these studies has explored maturity in terms of global supply chains and their response capacity amid impacting and unexpected events such as COVID-19. [...]this special issue sought to publish papers that clearly demonstrate the practical and theoretical impacts of maturity in global supply chains in the context of sudden disruptive events such as COVID-19. The second article, which is titled “A systematic review of AR/VR in operations and supply chain management: maturity, current trends and future directions” has brought a relevant contribution to the issue by providing a systematic insight into the current maturity of augmented reality (AR) and virtual reality (VR) in operations and supply chain management (OSCM), considering the context of COVID-19. The research findings demonstrated that AR/VR are still in the introduction and growth phase and that they have yet to reach their maturity. [...]the study has also shown that there is a limited utilization of AR/VR as drivers in facilitating sustainable practices in OSCM and that the potential applications of these technologies for the recovery phase of supply chains in the post-COVID-19 era require special attention. The five papers on this special issue have approached maturity management by considering the context of the COVID-19 pandemic from different perspectives and making the following contributions: determining the resilience and agility factors that increase the maturity of fast fashion supply chains;establishing how disruptive technologies such as virtual and AR can contribute to developing more resilient and mature supply chains;identifying research and practice gaps in supply chain resilience by connecting this topic with cost-effective and sustainable supply chain themes and showing how these key strategies enhance supply chain maturity and survivability;studying strategies focused on supply chain digitalization and integration for enhancing resilience in MOSCs amid a pandemic situation;and determining the impact of different industrial sectors from the lens of their maturity management to the performance response amid the COVID-19 pandemic.
ABSTRACT
Blood-testis barrier is body's innate mechanism to defend germ cells by creating a physical and immunological barrier. But some viral infections are known to evade it. As ACE2 and TMPRSS2 are present all over the body including male reproductive tract, it is worth exploring how coronavirus disease (COVID-19) could possibly affect male fertility. A review of literature was done using search engines like PubMed, Medline, Google Scholar, etc., and all the latest articles up to May 2021 were considered. Some studies have substantiated the presence of orchitis in COVID patients using semen and tissue samples. Though most studies report the absence of virus in testis, involvement of seminiferous tubules has been seen in pathological analysis suggesting defective spermatogenesis. This can be primarily attributed to inflammation and increased vascular permeability. Other factors that could affect male fertility are fever, autoimmune response, drugs, and erectile dysfunction. Male fertility is an important aspect of health care and must be looked into. Further studies can be done to understand host immunity towards SARS-CoV-2 in the testis. It will be worthwhile to know whether viral orchitis and its sequelae are acute or chronic in nature, and if they are reversible. Effect of the virus on female reproductive tract can also be assessed further. Counselling can be given to affected/recovering patients along with correct selection of drugs to prevent these long-term complications.
ABSTRACT
With the limitations imposed by COVID-19 pandemic, educational regulatory bodies in India recommended a number of online education portals to support higher education. Present work undertakes a detailed study on these recommended platforms and presents a comparative analysis based on the relevant features. 'SWAYAM' a widely popular and emphasized Indian MOOC platform has been compared with the other popular e-learning platforms of the international market. The study revealed that e-learning platforms vary in terms of offerings such as: method of dissemination of knowledge, target audiences, fee, target audiences, etc. Also, in terms of user base, courses offered, and certificates offered, 'SWAYAM' has to work a lot to compete with the international peers. Finally, SWOC analysis was carried out to present the threats and opportunities associated with recommended e-learning portals. The work will be profoundly helpful to frame the comprehensive education policies to meet the stakeholder's expectations and lead to the innovation of educational development.
ABSTRACT
Purpose The ongoing pandemic has gravely affected different facets of society and economic trades worldwide. During the outbreak, most manufacturing and service sectors were closed across the globe except for essential commodities such as food and medicines. Consequently, recent literature has focused on studying supply chain resilience and sustainability in different pandemic contexts. This study aims to add to the existing literature by exploring the economic, environmental and societal aspects affecting the food supply chain and assessing the impact of COVID-19 on food sustainability. Design/methodology/approach A survey method has been adopted with a questionnaire instrument investigating the role of technology, government policies, geopolitics and intermediaries on sustainable organisational management. A five-point Likert scale (i.e. 1 = strongly disagree;5 = strongly agree) is used to evaluate the responses. The findings are based on 131 responses from entry-level workers and senior executives of different food supply chains across Asia and Europe. The data has been analysed to derive insights into the impacts of this pandemic. Findings The survey concludes with the significant impact of COVID-19 on the three pillars of sustainability, i.e. economic, social and environmental dimensions. The empirical analysis shows digitalisation and its applications help mitigate the negative effect of COVID-19 on sustainability. In addition, the supportive government policies and intermediatory interventions were helpful in improving sustainability at each level. Research limitations/implications The findings have implications for businesses and policymakers. Companies can learn from the advantages of digitalisation to counter the challenges imposed by the pandemic or similar situations in the future in maintaining the sustainability of their supply chains. Managers can also learn the importance of effective organisational management in driving sustainability. Finally, policymakers can devise policies to support businesses in adopting sustainable practices in their supply chains. Originality/value This study adds to the limited literature exploring the impact of COVID-19 on food supply chain sustainability through the triple bottom line lens. To the best of the authors' knowledge, this is also one of the first empirical studies to examine the effect of technology, government and organisational management practices on the sustainability of food supply chains.
ABSTRACT
In light of recently increased e-commerce, also a result of the COVID-19 pandemic, this study examines how additive manufacturing (AM) can contribute to e-commerce supply chain network resilience, profitability and competitiveness. With the recent competitive supply chain challenges, companies aim to decrease cost performance metrics and increase responsiveness. In this work, we aim to establish utilisation policies for AM in a supply chain network so that companies can simultaneously improve their total network cost and response time performance metrics. We propose three different utilisation policies, i.e. reactive, proactive – both with 3D printing support – and a policy excluding AM usage in the system. A simulation optimisation process for 136 experiments under various input design factors for an (s, S) inventory control policy is carried out. We also completed a statistical analysis to identify significant factors (i.e. AM, holding cost, lead time, response time, demand amount, etc.) affecting the performance of the studied retailer supply chain. Results show that utilising AM in such a network can prove beneficial, and where the reactive policy contributes significantly to the network performance metrics. Practically, this work has important managerial implications in defining the most appropriate policies to achieve optimisation of supply network operations and resilience with the aid of AM, especially in times of turbulence and uncertainty.
ABSTRACT
Purpose - COVID-19 pandemic has shattered the economic systems all around the world while creating numerous problems which were faced by all, especially international migrants. The present study offers a qualitative and quantitative perspective on the distress of international migrants and their repatriation intention during the pandemic period. Design/methodology/approach - In-depth semi-structured interviews of 30 respondents belonging to five host nations, Australia, the USA, the UK, New Zealand and Canada, revealed diverse issues. Based on qualitative study findings and past literature, 22 purposeful statements about six constructs - financial issues, social issues, mobility constraints, psychological problems, healthcare issues, and repatriation intentions - were developed. These statements were measured on a seven-point Likert scale and shared online with international migrants from India residing in the host nations. Data collected from 496 international migrants from October 2020 to July 2021 were used to analyze the influence of various determinants on the repatriation intentions by partial least square-structural equation modeling using SmartPLS software. Findings - The analysis results revealed that the role of financial, social, mobility, psychological and healthcare issues was significant in strengthening the repatriation intentions of the migrants. There is a need to create job opportunities, retrain laid-off workers and formulate migrant inclusive policies. Originality/value - Although some studies have highlighted a few problems faced by international migrants, their impact on repatriation intentions has not been studied yet. The present study fills this gap and analyzes the repatriation intention of international migrants in light of different problems they faced during the pandemic.
ABSTRACT
Purpose This study aims to analyse the resilience of customer demand management post-coronavirus disease 2019, using fast fashion as an example. The paper provides insights for potential applications to micro-, small and medium enterprises (MSMEs). Design/methodology/approach Based on the qualitative analysis and an integrated Plan-Do-Check-Act (PDCA)-decision making trial and evaluation laboratory (DEMATEL)-fuzzy technique for order of preference by similarity to the ideal solution (TOPSIS) methodology of fuzzy multi-criteria decision-making, we explored and prioritised the enablers of resilience management for fast-fashion MSMEs. Findings The results reveal that the highest priority enabler is maintaining customer loyalty. Other enablers are associated with e-commerce endorsement, a customer-focussed assortment of items and flexible store operations. Research limitations/implications The study findings will enable fast-fashion MSMEs to develop effective actions and priorities in operations efforts to promote post-pandemic recovery. Originality/value Despite the importance of the resilience project and the changing fast-fashion customer patterns, only a handful of studies have explored how resilience can be managed in this field. Thus, the findings can contribute to closing this gap in the context of operations resilience research as well as MSME operations.
ABSTRACT
Pharmaceutical SC faces a great challenge due to COVID 19 restrictions and its impact on two of the largest manufacturers of pharmaceutical ingredients: Because of these restrictions, the non-delivery of pharma products to purchasing countries caused a shortfall and disturbance in pharmaceuticals SC. [...]logistics activities have ceased, cycle times have increased and the risk of product obsolescence has increased. [...]Taiwan used a health database and integrated it with the customs databases to begin creating big data for analysis and based on travel history and symptoms from clinical trials, real-time alerts were created regarding medical visits to help identify cases (Wang et al., 2020). [...]in the event of a pandemic like COVID-19, the resilience of the pharmaceutical and FSC is of paramount concern. [...]managing these operations is a top priority and addressing these issues requires proper design and management of SC stages such as planning, delivery, manufacturing and distribution.
ABSTRACT
COVID-19;Covishield;Covaxin;Immunization;Adverse Events Following Immunization INTRODUCTION COVID-19 also known as novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was declared Pandemic by the World Health Organization (WHO) and a Public Health Emergency of International Concern on January 30, 2020. Data Collection Pertinent medical history of the vaccinated population was collected, including name, age, sex, complete address, phone number, batch number, and date of vaccination, as well as the site of simultaneous vaccination. Description of various reported combined covishield first and second dose AEFI after vaccination is separated with the number of patients suffered with a particular adverse events- fever (75%), pain at injection site (32%), headache (23%), weakness (3%), body pain (29%), vomiting (4.5%), myalgia (2%), nausea (1%), cough (2.6%), red patches (2%), cramps (2%), chills (25%), diarrhea (3%), dizziness (7%), loss of appetite (6%), hypertension (0.52%), tachycardia (3%), flushing (1.2%), drowsiness (6%), restlessness (0.65%), stomach pain (2%), and fatigue (17%). [...]description of various reported combined covaxin first and second dose AEFI after vaccination is separated with the number of patients suffered with a particular adverse events - fever (63%), pain at injection site (30%), headache (22%), weakness (5%), body pain (50%), vomiting (2%), myalgia (2%), nausea (2.5%), cough (8%), red patches (2%), cramps (3.5%), chills (27%), diarrhea (2%), dizziness (5%), loss of appetite (4%), hypertension (2%), tachycardia (1.75%), flushing (1%), drowsiness (7%), restlessness (3%), stomach pain (4%), and fatigue (11%) illustrated in Figure 1.
ABSTRACT
The pandemic caused by SARS-CoV-2 (SCoV-2) has impacted the world in many ways and the virus continues to evolve and produce novel variants with the ability to cause frequent global outbreaks. Although the advent of the vaccines abated the global burden, they were not effective against all the variants of SCoV-2. This trend warrants shifting the focus on the development of small molecules targeting the crucial proteins of the viral replication machinery as effective therapeutic solutions. The PLpro is a crucial enzyme having multiple roles during the viral life cycle and is a well-established drug target. In this study, we identified 12 potential inhibitors of PLpro through virtual screening of the FDA-approved drug library. Docking and molecular dynamics simulation studies suggested that these molecules bind to the PLpro through multiple interactions. Further, IC50 values obtained from enzyme-inhibition assays affirm the stronger affinities of the identified molecules for the PLpro. Also, we demonstrated high structural conservation in the catalytic site of PLpro between SCoV-2 and Human Coronavirus 229E (HCoV-229E) through molecular modelling studies. Based on these similarities in PLpro structures and the resemblance in various signalling pathways for the two viruses, we propose that HCoV-229E is a suitable surrogate for SCoV-2 in drug-discovery studies. Validating our hypothesis, Mefloquine, which was effective against HCoV-229E, was found to be effective against SCoV-2 as well in cell-based assays. Overall, the present study demonstrated Mefloquine as a potential inhibitor of SCoV-2 PLpro and its antiviral activity against SCoV-2. Corroborating our findings, based on the in vitro virus inhibition assays, a recent study reported a prophylactic role for Mefloquine against SCoV-2. Accordingly, Mefloquine may further be investigated for its potential as a drug candidate for the treatment of COVID.
ABSTRACT
Neurological complications following SARS-CoV-2 infections are increasingly recognized. Despite that, sequelae from SARS-CoV-2 infection in gravid women are uncommonly reported. We present a case of acute motor axonal neuropathy, a variant of Guillain-Barre syndrome, in a 26-year-old primigravida at 34 weeks' gestation. She presented with worsening diplopia, dysphagia, and lower extremity sensory and motor deficits after testing positive for SARS-CoV-2 2 weeks earlier. Due to rapidly progressive symptoms, she required endotracheal intubation and mechanical ventilation. Initially she was diagnosed with Miller Fisher syndrome, but serology and electromyography studies were consistent with acute motor axonal neuropathy. Spontaneous preterm delivery, supportive care, and intravenous immunoglobulin therapy resulted in clinical improvement. Steady recovery was achieved by a prolonged rehabilitation program.